Immunodiffusion

Immunodiffusion

Immunodiffusion

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Immunodiffusion is a diagnostic test which involves diffusion through a substance such as agar[1] which is generally soft gel agar (2%) or agarose (2%), used for the detection of antibodies or antigen.

Quick Facts MeSH ...

The commonly known types are:

  1. Single diffusion in one dimension (Oudin procedure)
  2. Double diffusion in one dimension (Oakley Fulthorpe procedure)
  3. Single diffusion in two dimensions (radial immunodiffusion or Mancini method)[2]
  4. Double diffusion in two dimensions (Ouchterlony double immunodiffusion)[3]

Notes

  1. [1]
    Coleman, R. M.; Kaufman, L. (1972). "Use of the Immunodiffusion Test in the Serodiagnosis of Aspergillosis". Applied Microbiology. 23 (2): 301–308. doi:10.1128/am.23.2.301-308.1972. PMC 380335. PMID 4622826.
  2. [2]
    "Radial Immunodiffusion". Edvotek, Inc. 2017. Archived from the original (photograph) on 2017-08-07. Retrieved 2017-08-07. Photograph of precipitin circles in a Petri dish during radial immunodiffusion.
  3. [3]
    "Diffusion Patterns". Immunodiffusion principles and application. Archived from the original on 2019-12-11. Retrieved 2017-05-19. Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity.
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