The lacrimal canaliculi (sg.: canaliculus) are the small channels in each eyelid that drain lacrimal fluid, from the lacrimal puncta to the lacrimal sac. This forms part of the lacrimal apparatus that drains lacrimal fluid from the surface of the eye to the nasal cavity.^[1]

There is a single lacrimal canaliculus in each eyelid, a superior lacrimal canaliculus in the upper eyelid and an inferior lacrimal canaliculus in the lower eyelid. The canaliculi travel vertically and then turn medially to travel towards the lacrimal sac. At the bend, the canaliculus is dilated and called the ampulla. Usually, the superior and inferior lacrimal canaliculi join to form a common passage that enters the lateral wall of the lacrimal sac.

The lacrimal canaliculi have a mucosa composed of a non-keratinized stratified squamous epithelium on a basement membrane and a highly elastic lamina propria. Surrounding the mucosa are skeletal muscle fibres continuous with the orbicularis oculi which forms a sort of sphincter. This may facilitate the draining of lacrimal fluid during blinking.^[1]

Canaliculitis is inflammation of the lacrimal canaliculus.