Events from the year 1843 in Scotland.

• 18 May – the Disruption of the Church of Scotland takes place.^[1]
• 3 June – first burial in Warriston Cemetery, Edinburgh.
• 29 June – Robert Napier launches his first iron ship, the paddle steamer Vanguard, from his new yard at Govan on the River Clyde.^[2]
• 1 July – Union Bank of Scotland opens in Glasgow.
• 13 August – Sir William Dunbar, priest of St. Paul's Chapel, Aberdeen, is excommunicated from the Scottish Episcopal Church for refusing to administer or receive the sacrament in accordance with the church's ritual.
• Dingwall becomes the county town of Ross and Cromarty.
• The last laird of Raasay, John Macleod, emigrates to Tasmania having sold the Scottish island to George Rainy to help clear his debts.^[3]
• The Ordnance Survey commences its first published mapping of Scotland with a survey of Wigtownshire.^[4]
• The Glenmorangie distillery is established in Tain by William Matheson.
• Glenburn Hydro is opened in Rothesay, Bute, the first hydropathic establishment in Scotland.
• First paddle steamer on Loch Katrine, Gypsy.
• Little Ross lighthouse completed.
• Angus MacKay becomes first Piper to the Sovereign.
• Marion Kirkland Reid's feminist tract A Plea for Woman is published in Edinburgh.

• 12 June – David Gill, astronomer known for measuring astronomical distances, for astrophotography, and for geodesy (died 1914)
• 5 August – James Scott Skinner, dancing master, fiddler and composer (died 1927)
• 21 August – Thomas Hill Jamieson, librarian (died 1876)

• 25 July – Charles Macintosh, chemist and inventor of waterproof fabrics after whom the Mackintosh raincoat is named (born 1766)^[5]
• 5 December – David Hamilton, architect (born 1768)

• Hill & Adamson form Scotland's first photographic studio, on Calton Hill in Edinburgh.

• Timeline of Scottish history
• 1843 in Ireland