In enzymology, a 2,4'-dihydroxyacetophenone dioxygenase (EC 1.13.11.41) is an enzyme that catalyzes the chemical reaction

2,4'-dihydroxyacetophenone + O₂ ${\displaystyle \rightleftharpoons }$ 4-hydroxybenzoate + formate

Thus, the two substrates of this enzyme are 2,4'-dihydroxyacetophenone and O₂, whereas its two products are 4-hydroxybenzoate and formate.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O₂. The systematic name of this enzyme class is 2,4'-dihydroxyacetophenone oxidoreductase (C-C-bond-cleaving). This enzyme is also called (4-hydroxybenzoyl)methanol oxygenase. This enzyme participates in bisphenol a degradation.^[1]