The 13th Annual Nickelodeon Kids' Choice Awards was held on April 15, 2000, at the Hollywood Bowl in Los Angeles, live on Nickelodeon, with around 17,000 in attendance at the venue. Rosie O'Donnell hosted for the fifth consecutive year, along with LL Cool J, David Arquette, Mandy Moore, and Frankie Muniz as co-hosts. The show featured a SpongeBob SquarePants short to introduce the Favorite Cartoon nominees and announce the winner.

Over 15 million voters cast ballots for the ceremony, including for ten online-only categories.

• Goo Goo Dolls - "Broadway" & "Slide"
• Jennifer Lopez - Intro: "Let's Get Loud", "Feelin' So Good"
• *NSYNC - "Bye, Bye, Bye"
• Jessica Simpson & Nick Lachey - "Where You Are"

• David Arquette and Bill Goldberg - presented Favorite Movie Actor
• Kristen Johnston and Stephen Collins - presented Favorite Movie Actress
• 98 Degrees - introduced winners for Favorite Movie Couple and Favorite Voice From an Animated Movie
• Bullwinkle J. Moose - introduced Robert De Niro
• Robert De Niro - presented Favorite Movie
• Frankie Muniz and Julia Louis Dreyfuss - presented Favorite TV Actress
• Steve Burns, Leslie Bibb and Carly Pope - presented Favorite TV Friends
• SpongeBob SquarePants and Patrick Star - presented Favorite Cartoon
• Ray Romano - presented Favorite TV Show
• Roy Jones Jr. and John Francis Daley - presented Favorite MVP
• Mandy Moore and Nick Cannon - presented Favorite Male Singer
• Jamie Foxx - introduced winners for Favorite Female Artist, Favorite Song from a Movie, and Favorite Music Group
• Amanda Bynes and Mel Gibson - presented Favorite New Music Artist
• Lindsay Felton and Tyrese - presented Favorite Band
• LFO and Melissa Joan Hart - presented Favorite Song
• Jennifer Love Hewitt - presented The Hall Of Fame Award

Winners are listed first and in boldface.

• Rosie O'Donnell