The 2000–01 network television schedule for the six major English language commercial broadcast networks in the United States covers primetime hours from September 2000 through August 2001. The schedule is followed by a list per network of returning series, new series, and series cancelled after the 1999–2000 season.

PBS is not included; member stations have local flexibility over most of their schedules and broadcast times for network shows may vary.

New series are highlighted in bold.

All times are U.S. Eastern and Pacific Time (except for some live sports or events). Subtract one hour for Central, Mountain, Alaska and Hawaii-Aleutian times.

From September 15 to October 1, 2000, all of NBC's primetime programming was preempted in favor of coverage of the 2000 Summer Olympics in Sydney.

Each of the 30 highest-rated shows is listed with its rank and rating in parentheses (#rank / rating), as determined by Nielsen Media Research.^[1]

Note: UPN had acquired the Sunday evening XFL games from February 11 to April 15 (to compete with Fox's Sunday night lineup), while NBC broadcast Saturdays games till the end of the XFL season.

Note: On Fox, Schimmel supposed to air 8-8:30, but it was cancelled due to production problems.

Note: On Fox, an untitled Michael Crichton project was supposed to air at midseason at 9–10, along with The Lone Gunmen at 8-9 but that Crichton project for Fox was scrapped.

NOTE: ABC aired Dot Comedy on December 8, 2000, at 8:30pm ET. The show was cancelled after one episode aired. On Fox, Night Visions was supposed to air 8–9, but it was delayed to summer in a different timeslot on Thursdays.