The 2010 Tercera División play-offs to Segunda División B from Tercera División (Promotion play-offs) were the final playoffs for the promotion from 2009–10 Tercera División to 2010–11 Segunda División B. The first four teams in each group (excluding reserve teams) took part in the play-off.

The eighteen group winners had the opportunity to be promoted directly to Segunda División B. The eighteen group winners were drawn into a two-legged series where the nine winners promoted to Segunda División B. The nine losing clubs entered the play-off round for the last nine promotion spots.

The eighteen runners-up were drawn against one of the seventeen fourth-placed clubs outside their group and the eighteen third-placed clubs were drawn against one another in a two-legged series. The twenty-seven winners advanced with the nine losing clubs from the champions' series to determine the eighteen teams that entered the last two-legged series for the last nine promotion spots. In all the playoff series, the lower-ranked club played at home first. Whenever there was a tie in position (e.g. like the group winners in the champions' series or the third-placed teams in the first round), a draw determined the club to play at home first.

• All groups as 38 of 38 rounds.
• The teams highlighted in yellow played the play-offs to Segunda División B.
• The teams highlighted in red were relegated to Divisiones Regionales.

• The regular season ended on the 9 May 2010.
• The draw of play-offs were held in the RFEF headquarters on 10 May at 17:30. (CEST+2).
• The play-offs began on the 15 May and will end on the 20 June 2010.

• 2010 Segunda División B play-offs

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