The 2013–14 Czech Women's First League is the 21st season of the Czech Republic's top-tier football league for women. Sparta Praha were the defending champions. For the first time two teams qualify to the Champions League, because the Czech Republic entered the top eight nations in the UEFA Coefficients for women.

The championship was won by Slavia for the third time, and the first time since 2004. Slavia's win over Sparta at the penultimate matchday ended their nine-year-long title streak.

The eight teams will play each other twice for a total of 14 matches per team. After that the top four teams will play a championship round for another six matches per team. The bottom placed four teams play the relegation round. Points accumulated after the regular season are halved and added the points from the next round. The champion and runners-up qualify for the UEFA Women's Champions League.

Points of the regular season were halved and rounded up, goal difference was kept.

Note: Flags indicate national team as has been defined under FIFA eligibility rules. Players may hold more than one non-FIFA nationality.

More information Team, Manager ...

Team	Manager	Captain	Kit manufacturer	Shirt sponsor
Baník Ostrava	Aleš Döme	Taťána Michnová	Nike	Deník
Hradec Králové	Petr Kincl	Veronika Benešová	Adidas
Slavia Praha	Anton Mišovec	Blanka Pěničková	Umbro
Slovácko	Petr Vlachovský	Petra Polášková	Kappa	Neoklas
Sparta Praha	Jan Podolák	Iva Mocová	Nike	Fortuna
Viktoria Plzeň	Karel Fajfrlík	Eva Bohmannová	Alea
Bohemians Praha	Jiří Dědič	Lucia Ondrušová	Adidas	GHC Genetics
Pardubice	Petr Šmeral	Kateřina Urbancová	Jako

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