ATFS may refer to:

• Artificial transcription factor, an example of a chimeral protein designed to target and modulate gene transcription.
• Association of Track and Field Statisticians, an international organisation run by volunteers whose goal is to collect and disseminate the statistics of Track and Field Athletics.
• After The Forking Show, the explicit podcast created by the team from Spoonman's Classic Rock Show.
• American Tree Farm System, a certification program for tree farms

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