Anna Sophie Charlotte of Brandenburg-Schwedt (24 December 1706 – 3 January 1751), was a German noblewoman and member of the House of Hohenzollern and by marriage Duchess of Saxe-Eisenach.

Born in Berlin, she was the third of seven children born from the marriage of Margrave Albert Frederick of Brandenburg-Schwedt (a younger brother of King Frederick I of Prussia) and Maria Dorothea Kettler, by birth Princess of Courland and Semigallia. From Anna Sophie's six older and younger siblings, only four survive adulthood: three brothers (Charles Frederick Albert, Frederick and Frederick William; all died unmarried and without legitimate issue) and one sister (Sophie Fredericka Albertine, by marriage Princess of Anhalt-Bernburg).

In Berlin on 3 June 1723 Anna Sophie married Wilhelm Heinrich, Hereditary Prince of Saxe-Eisenach as his second wife. They had no children.

She became in Duchess consort of Saxe-Eisenach after the accession of her husband on 14 January 1729.

Anna Sophie died in Sangerhausen aged 44, having survived her husband by nine years. She was buried in Halle.