Final portions/segments

• Aquiknow and Aboonduh Tonite - A parody of defunct late-night talk show Aquino & Abunda Tonight hosted by Kris Aquino and Boy Abunda. This portion is played by Angelica Panganiban as Krissy Aquiknow, and Jayson Gainza as Bhoy Aboonduh.
• Krissy TV - A parody of defunct morning talk show Kris TV hosted by Kris Aquino. It is portrayed by Angelica Panganiban as Krissy Aquiknow, Jayson Gainza as Krissy's assistant Dharla (a parody of Kris TV headwriter Darla Sauler), and John Prats as Bimby Aquino Yap.
• Clown in a Million - The talent search for comedians, where funny people with no or little television experience battle it out to be proclaimed as the latest comedic talent discovery.
• Banana Classics - A sketch or comic skit where two or more actors portray roles to convey a simple story that culminates in a punchline, just to make people laugh. It is done before a live audience without cuts, thus requiring the material to be visual rather than verbal.
• Extra Spoof - A segment where the cast impersonates famous icons, idols, and celebrities of past and present times, living or deceased. It is cohesively presented with a common theme like novelty icons, the icons from the 80s, 90s, and 2000s, or internationally known Pinoy singers.
• Make Me Rap -
• Oh My Shopping! - A parody of O Shopping where the cast is asked to pick a certain item from a chosen box which they need to sell like an infomercial show.

Former segments (from Banana Nite and Banana Sundae)

• Barado Sisters (later as "Hala Bara!")
• Eto na Post
• Kandila (parody of late-night newscast Bandila)
  • Ihaw Na! (parody of Ikaw Na!, a defunct Bandila segment)
• Krissy and Me (parody of Kris TV)
• Laugh Three
• "Painting"
• Surprise Bwi-set
• Tres Kumpares
• Rated SSPG: Sobra-Sobrang Pilyong Gags, Bawal Sa Bata, Tulog Na! (a parody of MTRCB's SPG rating advisory, a collection of green jokes)
• Chazz Do It
• Yaya and Angel (parody of Ang Spoiled, a sketch from rival gag show Bubble Gang)
• Mr. Bang (parody of Mr. Bean)
• Aquiknow & Aboonduh Tonite (parody of Aquino & Abunda Tonight)
• Sosyal, 'Di Sosyal
• Banana Recap
  • Monday Funday
  • Timeless Tuesday
  • Wayback Wednesday
  • Throwback Thursday
  • Flashback Friday
• Maling Akala
• Dr. Selfie
• Balitang Six-Six
• Fake Yan
• Pag May Time
• Kontra Pick-Up
• Pinoy True Stories parodies
  • TnT: Tapatan ni Tunting (parody of Tapatan ni Tunying)
  • Bistodo (parody of Bistado)
  • Kutya ng Masa (parody of Mutya ng Masa)
• How Dare You (parody of reality show I Dare You)
• Single, In a Relationship, It's Complicated
• Munting Kaalaman
• Pogi vs. Tambay
• Hot Issue
• Brad Bro Bru
• Isang Tanong, Isang Sagot

Former portions/segments

Elimination chart

  The contestant won the competition

  The contestant was eliminated

  The contestant won the wild card round.

  The contestant won immunity from the next elimination

  The contestant was deemed the worst but was immune from elimination

Theme song

Lito Camo composed the theme song Banana Split performed by Kool Chix, and is available as a bonus track in Willie Revillame's album Giling Giling from Star Records. However, the theme song was changed during the third season, which is a re-composition of The Champs' Tequila.^[10]

Gay kiss issue

During the December 6, 2008 episode, Rafael Rosell (as a guest), Jon Avila, and Will Devaughn were involved in a joke segment following a dating game, where Rosell was kissed by the gay actors instead of the usual Banana Split girls. Rosell consented to the practical joke and to being blindfolded and kissed on the cheek, but they instead kissed him on the lips. This event caused a short rift between Rosell and a Banana Split executive producer, as Rosell thought he was being taken advantage of. As a result, few clips were aired instead of the whole segment. A public apology was also delivered after the end of the show.^{[11][12][13][14]}

Casting cut-out

This section may be confusing or unclear to readers. (June 2011)

Valerie Concepcion, Roxanne Guinoo and Dianne Medina left Banana Split to focus on Precious Hearts Romances series where they will act in different episodes. It was reported that Guinoo was removed from the show because of her weight problems,^[15] while Concepcion left the show due to a talent fee dispute.^[16] The rift between Angelica Panganiban and Cristine Reyes arose as another issue, resulting in Reyes' departure.^[17]

John Prats and Jason Francisco Altercation

Cast members John Prats and Jason Francisco figured in an altercation during rehearsals for a show in 2013 to be held at the Music Museum.^[18] The two parties eventually patched things up a year later.^[19]

Binay Blackface skit

Banana Sundae attracted controversy prior to the May 13, 2019, elections when they aired a skit parodying the church argument of Binay siblings Abby Binay and Junjun Binay, who were both Makati mayoral candidates. Pooh and former SexBomb Girls member Sunshine Garcia played as the Binay siblings, putting black makeup on themselves. The skit drew mixed reactions with viewers calling it "racist and insensitive" as well as referencing the history of blackface, while others noted that the show never mocked skin color, but instead, the argument itself and the political history of the Binay family.^[20]