CHES_(buffer)

CHES (buffer)

CHES (buffer)

Chemical compound

CHES (N-cyclohexyl-2-aminoethanesulfonic acid) is a buffering agent. CHES buffers have a useful range of pH 8.6–10.[1]

Quick Facts Names, Identifiers ...

It typically appears as a white crystalline powder.

Effect of impurities

Commercial prep of CHES (and other sulfonylethyl buffers like MES, BES, and PIPES) can contain a contaminant oligo(vinylsulfonic acid) (OVS), which is a polyanionic mimic of RNA, and can be a potent (pM) inhibitor of RNA binding proteins and enzymes.[2]

References

  1. [1]
    "Good's buffers (biological buffers)" (PDF).
  2. [2]
    Smith, Bryan D.; Soellner, Matthew B.; Raines, Ronald T. (2003). "Potent Inhibition of Ribonuclease A by Oligo(vinylsulfonic Acid)". Journal of Biological Chemistry. 278 (23). Elsevier BV: 20934–20938. doi:10.1074/jbc.m301852200. ISSN 0021-9258.


Stub icon

This article about an organic compound is a stub. You can help Wikipedia by expanding it.
Share this article:

This article uses material from the Wikipedia article CHES_(buffer), and is written by contributors. Text is available under a CC BY-SA 4.0 International License; additional terms may apply. Images, videos and audio are available under their respective licenses.