For other uses, see Confluence (disambiguation).

In cell culture biology, confluence refers to the percentage of the surface of a culture dish that is covered by adherent cells. For example, 50 percent confluence means roughly half of the surface is covered, while 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer.[1] The cell number refers to, trivially, the number of cells in a given region.

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