In enzymology, a cyclamate sulfohydrolase (EC 3.10.1.2) is an enzyme that catalyzes the chemical reaction

cyclohexylsulfamate + H₂O ${\displaystyle \rightleftharpoons }$ cyclohexylamine + sulfate

Thus, the two substrates of this enzyme are cyclohexylsulfamate and H₂O, whereas its two products are cyclohexylamine and sulfate.

This enzyme belongs to the family of hydrolases, specifically those acting on sulfur-nitrogen bonds. The systematic name of this enzyme class is cyclohexylsulfamate sulfohydrolase. Other names in common use include cyclamate sulfamatase, cyclamate sulfamidase, and cyclohexylsulfamate sulfamidase. This enzyme participates in caprolactam degradation.