"Don't Tread on Me" is a song by We the Kingdom that was released as the third single from their debut studio album, Holy Water (2020), on April 24, 2020.^[1] The song was written by Ed Cash, Scott Cash, Franni Cash, Martin Cash, and Andrew Bergthold.

"Don't Tread On Me" peaked at No. 40 on the US Hot Christian Songs chart.^[2]

"Don't Tread on Me" was released by We the Kingdom as the third single from their debut studio album, Holy Water (2020), along with its accompanying lyric video.^[1] Ed Cash shared the message of the song,^[3] saying:

It's one of those songs, that for us, is just a powerful thing to proclaim. We've been given authority over the kingdom of darkness by Jesus. When we come together and we proclaim that, it is really powerful.

"Don't Tread On Me" is composed in the key of E minor with a tempo of 70 beats per minute and a musical time signature of ⁴
₄.^[4] The singers' vocal range spans from D₄ to C₅.^[5]

"Don't Tread On Me" made its debut at No. 40 on the US Hot Christian Songs chart dated August 22, 2020,^[6] following its commercial release.

We the Kingdom released the lyric video of "Don't Tread On Me" on April 24, 2020.^[7] The live music video of the song, recorded at Young Life Sharptop Cove in Jasper, Georgia, was released on May 5, 2020, on YouTube.^[8][9] The Church Sessions video featuring an appearance by Maverick City Music was released on October 19, 2020.^[10] The Storybrooke Sessions video was released to YouTube on June 9, 2020.^[11][12]