Dysferlin also known as dystrophy-associated fer-1-like protein is a protein that in humans is encoded by the DYSF gene.^[5] Dysferlin is linked with plasma membrane repair.,^[6] stabilization of calcium signaling^[7][8][9] and the development of the T-tubule system of the muscle^[10] A defect in the DYSF gene, located on chromosome 2p12-14, results in several types of muscular dystrophy; including Miyoshi myopathy (MM), Limb-girdle muscular dystrophy type 2B (LGMD2B) and Distal Myopathy (DM). A reduction or absence of dysferlin, termed dysferlinopathy, usually becomes apparent in the third or fourth decade of life and is characterised by weakness and wasting of various voluntary skeletal muscles.^[11] Pathogenic mutations leading to dysferlinopathy can occur throughout the DYSF gene.

Quick Facts DYSF, Available structures ...

DYSF
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4CAH, 4CAI, 4IHB, 4IQH
Identifiers
Aliases	DYSF, FER1L1, LGMD2B, MMD1, dysferlin, LGMDR2
External IDs	OMIM: 603009 MGI: 1349385 HomoloGene: 20748 GeneCards: DYSF
Gene location (Human) Chr. Chromosome 2 (human)[1] Band 2p13.2 Start 71,453,561 bp[1] End 71,686,763 bp[1]
Gene location (Mouse) Chr. Chromosome 6 (mouse)[2] Band 6 C3|6 36.14 cM Start 83,985,572 bp[2] End 84,188,042 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in blood triceps brachii muscle vastus lateralis muscle external globus pallidus gastrocnemius muscle deltoid muscle spleen right ventricle tibialis anterior muscle left ventricle Top expressed in soleus muscle skeletal muscle tissue interventricular septum yolk sac triceps brachii muscle temporal muscle quadriceps femoris muscle digastric muscle myocardium of ventricle gastrocnemius muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function calcium ion binding metal ion binding protein binding calcium-dependent phospholipid binding phospholipid binding lipid binding microtubule binding alpha-tubulin binding Cellular component integral component of membrane endosome late endosome membrane T-tubule plasma membrane endocytic vesicle early endosome sarcolemma extracellular exosome cytoplasmic vesicle membrane cytoplasmic vesicle lamellipodium microtubule organizing center nucleus Golgi apparatus microtubule membrane microdomain Biological process muscle contraction plasma membrane repair vesicle fusion angiogenesis positive regulation of endothelial cell proliferation monocyte activation involved in immune response macrophage activation involved in immune response glycerol metabolic process negative regulation of gene expression lipid storage T-tubule organization negative regulation of protein catabolic process skeletal muscle tissue regeneration fat cell differentiation positive regulation of cell adhesion negative regulation of phagocytosis cellular response to osmotic stress positive regulation of neutrophil chemotaxis regulation of calcium ion import negative regulation of high voltage-gated calcium channel activity negative regulation of protein polyubiquitination Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8291 26903 Ensembl ENSG00000135636 ENSMUSG00000033788 UniProt O75923 Q9ESD7 RefSeq (mRNA) NM_001130455 NM_001130976 NM_001130977 NM_001130978 NM_001130979 NM_001130980 NM_001130981 NM_001130982 NM_001130983 NM_001130984 NM_001130985 NM_001130986 NM_001130987 NM_003494 NM_001077694 NM_021469 NM_001310152 RefSeq (protein) NP_001123927 NP_001124448 NP_001124449 NP_001124450 NP_001124451 NP_001124452 NP_001124453 NP_001124454 NP_001124455 NP_001124456 NP_001124457 NP_001124458 NP_001124459 NP_003485 NP_001071162 NP_001297081 NP_067444 NP_001390584 NP_001390585 NP_001390586 NP_001390587 Location (UCSC) Chr 2: 71.45 – 71.69 Mb Chr 6: 83.99 – 84.19 Mb PubMed search [3] [4]
Wikidata
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The human dysferlin protein is a 237 kilodalton type-II transmembrane protein.^{[12][13][14][15][16]} It contains a large intracellular cytoplasmic N-terminal domain, an extreme C-terminal transmembrane domain, and a short C-terminal extracellular domain. The cytosolic domain of dysferlin is composed of seven highly conserved C2 domains (C2A-G) which are conserved across several proteins within the ferlin family, including dysferlin homolog myoferlin.^[17][18][13] In fact, the C2 domain at any given position is more similar to the C2 domain at the corresponding position within other ferlin family members than the adjacent C2 domain within the same protein. This suggests that each individual C2 domain may in fact play a specific role in dysferlin function and each has in fact been shown to be required for two of dysferlin's roles stabilization of calcium signaling and membrane repair.^[19] Mutations in each of these domains can cause dysferlinopathy. A crystal structure of the C2A domain of human dysferlin has been solved, and reveals that the C2A domain changes conformation when interacting with calcium ions,^[13] which is consistent with a growing body of evidence suggesting that the C2A domain plays a role in calcium-dependent lipid binding.^[20] Its ability to stabilize calcium signaling in the intact dysferlin protein depends on its calcium binding activity.^[21] In addition to the C2 domains, dysferlin also contains "FerA" and "DysF" domains. Mutations in both FerA^[22] and DysF^[23] can cause muscular dystrophies. DysF domain has an interesting structure as in contains one DysF domain within another DysF domain, a result of gene duplication; however, the function of this domain is currently unknown.^[23] FerA domain is conserved among all members of ferlin protein family. FerA domain is a four helix bundle and it can interact with membrane, usually in a calcium-dependent manner.^[22]

The most intensively studied role for dysferlin is in a cellular process called membrane repair. Membrane repair is a critical mechanism by which cells are able to seal dramatic wounds to the plasma membrane. Muscle is thought to be particularly prone to membrane wounds given that muscle cells transmit high force and undergo cycles of contraction. Dysferlin is highly expressed in muscle, and is homologous to the ferlin family of proteins, which are thought to regulate membrane fusion across a wide variety of species and cell types.^[24] Several lines of evidence suggest that dysferlin may be involved in membrane repair in muscle. First, dysferlin-deficient muscle fibers show accumulation of vesicles (which are critical for membrane repair in non-muscle cell types) near membrane lesions, indicating that dysferlin may be required for fusion of repair vesicles with the plasma membrane. Further, dysferlin-deficient muscle fibers take up extracellular dyes to a greater extent than wild-type muscle fibers following laser-induced wounding in-vitro.^[25] Dysferlin is also markedly enriched at membrane lesions with several additional proteins thought to be involved in membrane resealing, including annexin and MG53.^[26] Exactly how dysferlin contributes to membrane resealing is not clear, but biochemical evidence indicates that dysferlin may bind lipids in a calcium-dependent manner, consistent with a role for dysferlin in regulating fusion of repair vesicles with the sarcolemma during membrane repair.^[27] Furthermore, live-cell imaging of dysferlin-eGFP expressing myotubes indicates that dysferlin localizes to a cellular compartment that responds to injury by forming large dysferlin-containing vesicles, and formation of these vesicles may contribute to wound repair.^[28] Dysferlin may also be involved in Alzheimer's disease pathogenesis.^[29]

Another well studied role for dysferlin is in stabilization of calcium signaling, especially following a mild injury. This approach was based on two observations: that muscle lacking dysferlin that is injured by eccentric contractions can repair its plasma membrane, or sarcolemma, as efficiently as healthy muscle can,^[30] and that most of the dysferlin in healthy muscle is concentrated in the transverse tubules at triad junctions,^[31][32] where calcium release is regulated. Destabilization of signaling in dysferlinopathic muscle can result in the generation of calcium waves,^[33] which can contribute to the disease pathology. Nearly every change in dysferlin that affects membrane repair also destabilizes calcium signaling,^[34] suggesting that these two activities are closely linked. Remarkably, however, membrane repair requires calcium ions, whereas calcium ions contribute to the destabilization of signaling when dysferlin is absent or mutated.^[35] These paradoxical results have yet to be reconciled.

Dysferlin has been shown to bind to itself, to form dimers and perhaps larger oligomers.^[36] It can also has been shown to interact with Caveolin 3 in skeletal muscle,^[37] and this interaction is thought to retain dysferlin within the plasma membrane.^[38] Dysferlin also interacts with MG53, and a functional interaction between dysferlin, caveolin-3 and MG53 is thought to be critical for membrane repair in skeletal muscle.^[39]