Depth_Coded_Phalloidin_Stained_Actin_Filaments_Cancer_Cell.png

Summary

Description	English: A z-projection of an osteosarcoma cell, stained with phalloidin to visualise actin filaments. The image was taken on a confocal microscope, and the subsequent deconvolution was done using an experimentally derived point spread function. Image Parameters Microscope: Zeiss LSM 780 Confocal Microscope Pinhole [m]: 23 µm Total Size-Width: 133.69 µm Total Size-Height: 134.91 µm Total Size-Depth: 6.48 µm Voxel-Width: 42.0 nm (pixel size) Voxel-Height: 42.0 nm (pixel size) Voxel-Depth: 80.0 nm Resolution: 23.5938 pixels per micron Zoom: 1.0 Objective: Plan-Apochromat 63x/1.40 Oil DIC Numerical aperture: 1.40
Date	30 June 2014
Source	Own work
Author	Howard Vindin


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