University of Illinois

In 2003, Jillian Clarke of the University of Illinois at Urbana–Champaign found in a survey that 56% of men and 70% of women surveyed were familiar with the five-second rule. She also determined that a variety of foods were significantly contaminated by even brief exposure to a tile inoculated with E. coli.^[9] On the other hand, Clarke found no significant evidence of contamination on public flooring.^[9] For this work, Clarke received the 2004 Ig Nobel Prize in public health.^[10] A more thorough study in 2007 using salmonella on wood, tiles, and nylon carpet, found that the bacteria could thrive under dry conditions even after twenty-eight days.^[11] Tested on surfaces that had been contaminated with salmonella eight hours previously, the bacteria could still contaminate bread and baloney lunchmeat in under five seconds. But a minute-long contact increased contamination about tenfold (especially on tile and carpet surfaces).^[12]

Rutgers University

Researchers at Rutgers University debunked the theory in 2016 by dropping watermelon cubes, gummy candies, plain white bread, and buttered bread from a height of five inches (13 cm) onto surfaces slathered in Enterobacter aerogenes. The surfaces used were carpet, ceramic tile, stainless steel and wood. The food was left on the surface for intervals of 5, 30 and 300 seconds. The scientists assessed the amount of E. aerogenes transferred between surface and food. Since bacteria tended to be attracted to moisture, wet food had more risk to have bacteria transferred than dry food. To the surprise of the researchers, carpet transferred fewer bacteria than steel or tile. Wood was hard to pin down as it showed a large variation. "The five-second rule is a significant oversimplification of what actually happens when bacteria transfer from a surface to food," Donald Schaffner, a Rutgers University biologist and an author of the research, stated in the Washington Post, "Bacteria can contaminate instantaneously."^[13]

A pediatrician criticized the study for discounting the danger in consuming food after one touches other surfaces like refrigerator handles, light switches, and wallets, which have been found to be similarly contaminated with bacteria.^[14]

Other

A 2006 study at Clemson University was unable to verify the rule, when looking at tile, wood, and carpet floors. It indicated that bacteria can survive on the ground for a long time.^[15]

A 2014 study by biology students at Aston University in England suggested that there may be a basis for the five-second rule.^[16] Anthony Hilton, head of microbiology at Aston University, indicated in 2017 that food dropped on a seemingly clean floor for a few moments can be eaten with minimal risk.^[17] According to Hilton, moist foods that are left on the floor for more than 30 seconds are contaminated with 10 times more bacteria than food that has been left on the floor for 3 seconds.^[18]

Informal

The five-second rule was featured in an episode of the Discovery Channel series MythBusters, which discovered that there was no significant difference in the number of bacteria collected. The aspects that affect the contamination process is the moisture, surface geometry and the location.^[19] An episode of Food Detectives found that bacteria will cling to food immediately.^[20]