The women's football tournament at the 2000 Summer Olympics in Sydney was held from 13 to 28 September 2000. The women's tournament was a full international tournament with no restrictions on age. The eight national teams involved in the tournament were required to register a squad of 18 players, including two goalkeepers. Additionally, teams could name a maximum of four alternate players, numbered from 19 to 22. The alternate list could contain at most three outfielders, as at least one slot was reserved for a goalkeeper. In the event of serious injury during the tournament, an injured player could be replaced by one of the players in the alternate list. Only players in these squads were eligible to take part in the tournament.

The age listed for each player is on 13 September 2000, the first day of the tournament. The numbers of caps and goals listed for each player do not include any matches played after the start of the tournament. The club listed is the club for which the player last played a competitive match prior to the tournament. A flag is included for coaches who are of a different nationality than their own national team.