First and second patrols

She departed the German island on 1 March 1942, crossed the North Sea entered the Atlantic Ocean via the Faroe / Shetland gap and headed for the US east coast. Her first victim was Equipoise, sunk on 27 March 1942 60 nautical miles (110 km; 69 mi) southeast of Cape Henry, Virginia. The confusion of the sinking was not helped by there being nationals from at least ten countries among the crew. The boat went on to successfully attack City of New York, Rio Blanco and Ulysses. One ship that did not sink was Bidwell; indeed, she survived the war, not being broken up until 1965.

U-160's second foray saw the boat leave Lorient on 20 June 1942. She crossed the Atlantic again but made for the northern coast of South America. The pickings were just as rich here as they had been further north. Sinking Beaconlight, Carmona and the Treminnard, who were all sailing without an escort, was accomplished within 200 nmi (370 km; 230 mi) of Trinidad. She also damaged Thorshavet, an 11,015 GRT tanker, with torpedo and gun on 4 August 1942. The drifting wreck was subsequently sunk by the Italian submarine Enrico Tazzoli on 6 August. U-160 returned to Lorient on the 24th.

Third patrol

It was during her third patrol that the boat almost came to grief. She sank HMS Castle Harbour, which was travelling as part of Convoy TRIN-19 from Trinidad at 2120 hours on 16 October 1942. The ship sank within twenty seconds with the loss of nine of her twenty-two crew.^[3][4] U-160 herself was attacked by the escorts of the convoy, but the damage was slight. The submarine returned to her former hunting grounds off South America and sank Gypsum Express and Leda to name but two.

Fourth patrol

Her fourth sortie was her longest, at 125 days, but also her most successful. Moving into the south Atlantic, she sank SS Roger B. Taney on 8 February. She then attacked and sank Nirpura, Empire Mahseer and Marietta E. east of South Africa. Also lost with Marietta E. were eight landing craft. Other ships were also sunk. When the submariners questioned the survivors of Aelbryn, they misunderstood the ship's name, reporting it as Arian, an American vessel.

Fifth patrol and loss

By now she was based in Bordeaux, from which she departed on 29 June 1943. She was sunk by TBM Avenger and F4F Wildcat aircraft from the carrier USS Santee south of the Azores on 14 July 1943 with the loss of all 57 on board.