2017

Holloway showed incredible range by competing in the long jump and the 4x400m relay, in addition to his hurdles specialty. He went undefeated in the 60m high hurdles, and won the NCAA Indoor Title in a collegiate-leading 7.58 seconds. He also finished 11th in the long jump and ran the second leg on Florida's 4x400m relay team that finished 2nd. Outdoors, he won the 110m hurdles, finished 2nd in the long jump and anchored Florida's 4x400m relay team to 4th place. He also ran the 3rd leg on their 4x100m team throughout the season. Holloway competed in the US Championships in the hopes of qualifying for the World Championships in London, but finished 4th and missed out by .05 seconds.

2018

Holloway's 2018 season was a repeat of 2017. He continued his unbeaten streak in the 60m high hurdles, defending his NCAA title in a collegiate record-breaking and world leading 7.42 seconds. This year, he finished 2nd in the long jump and Florida finished 3rd in the 4x400m relay with his help on the second leg. Outdoors, he set a then-world-leading 13.15 seconds in the 110m hurdles at the SEC Championships, and defended his NCAA title 4 weeks later. He finished 9th in the long jump, ran the 2nd leg on the 4x100m team that finished 3rd, and anchored Florida to 4th place in the 4x400m. He once again went to the US Championships, this time finishing 2nd by thousandths of a second.

Prior to setting the NCAA and American record in the 60 m hurdles, in February 2018 he set the NCAA record with a 7.42 s clocking at the Clemson Tiger Paw Invitational, beating and taking the record away from Olympic champion in the 110 m hurdles Omar McLeod.^[7][16] Later that year he clocked 13.15 s in the 110 m hurdles at the Southeastern Conference (SEC) Championships, winning the meet in the second fastest time in NCAA history.^[17][18] This became the third fastest time a year later at the same meet when Holloway ran 13.07 s, just 0.07 s slower than Renaldo Nehemiah's NCAA record.^[19]

2019

2019 was Holloway's breakthrough year. Despite finding strong challenge from Daniel Roberts of the University of Kentucky, he went unbeaten in the 60m high hurdles and won his 3rd consecutive NCAA title in the event, becoming the first to do so. He also smashed his own collegiate record with 7.35 seconds, also an American record.

He was one of the most versatile athletes in the NCAA, setting personal bests of 6.50 s in the 60 m dash, 12.98 s in the 110 m hurdles, 8.17 m in the long jump, and a 43.75 s split in the 4 × 400 m relay while competing for the Florida Gators. He holds the NCAA and American record in the 60 m hurdles with a time of 7.35 s, set at the NCAA Division I Indoor Championships. This mark also made him the third-fastest man in the event in history.^[9][20][10] His NCAA record of 12.98 s in the 110 m hurdles, set at the NCAA Division I Championships, broke 40-year-old record held by former world record holder Renaldo Nehemiah.^[21] He was a member of the championship and record breaking 4 × 100 m relay team at this championships, which clocked the first sub-38 relay in NCAA history with a time of 37.97 s.^[22]

Holloway was NCAA champion in both the 60 m hurdles and the 110 m hurdles from 2017 to 2019, champion in both the 60 m dash and 60 m hurdles in 2019, and had multiple podium finishes in the long jump, 4 × 100 m relay, and 4 × 400 m relay in the same years. His performances were critical in helping the Florida Gators win the team titles at the 2017 NCAA Division I Championships, the 2018 NCAA Division I Indoor Championships, and the 2019 NCAA Division I Indoor Championships.