Greek National Road 1 (Greek: Εθνική Οδός 1, abbreviated as EO1) is the old single carriageway road connecting Athens with Thessaloniki and Evzonoi, the border crossing between Greece and North Macedonia. For most of its length, it has been replaced by the new A1 motorway.

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Quick Facts National Road 1, Major junctions ...

National Road 1
Εθνική Οδός 1
Major junctions
South end	central Athens
North end	North Macedonia border at Evzonoi
Location
Country	Greece
Regions	Attica, Central Greece, Thessaly, Central Macedonia
Major cities	Athens, Lamia, Larissa, Katerini, Alexandreia, Polykastro
Highway system
Highways in Greece Motorways National Roads

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According to the Government Gazette in 1963, the original alignment of EO1 ran from Athens to Evzoni and the international border with what was then Yugoslavia, via: Decelea, Sfendali (for Malakasa), Martino, Atalanti, Kamena Vourla, Thermopylae, Lamia, Stylida, Almyros, Velestino (for Volos), Larissa, Tempe, Katerini, Alexandreia, Chalkidona, Gefyra and Polykastro.^[1]

Today, the A1 motorway has absorbed most of EO1's original alignment, although the EO1 continues to run alongside most of the A1 for non-motorway traffic. However, there is a motorway-only gap west of Lake Yliki, requiring non-motorway traffic to detour via Thebes and Aliartos on the EO3. Additionally, the border crossing itself, north of Evzoni, is only open to motorway traffic.