In enzymology, a hexadecanol dehydrogenase (EC 1.1.1.164) is an enzyme that catalyzes the chemical reaction

hexadecanol + NAD⁺ ${\displaystyle \rightleftharpoons }$ hexadecanal + NADH + H⁺

Thus, the two substrates of this enzyme are hexadecanol and NAD⁺, whereas its 3 products are hexadecanal, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD⁺ or NADP⁺ as acceptor. The systematic name of this enzyme class is hexadecanol:NAD⁺ oxidoreductase.