INCENP

INCENP

INCENP

Protein-coding gene in the species Homo sapiens

Inner centromere protein is a protein that in humans is encoded by the INCENP gene.[5][6][7] It is a regulatory protein in the chromosome passenger complex (CPC). It is involved in regulation of the catalytic proteins Aurora B and Aurora C.[8][9][10] It acts in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B.[11][10]

Quick Facts Available structures, PDB ...
Quick Facts Chromosome passenger complex (CPC) protein INCENP N terminal, Identifiers ...
Quick Facts Inner centromere protein, ARK binding region, Identifiers ...

In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins.[12] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.

The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle.[13] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs),[5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis.[7][14]

Interactions

INCENP has been shown to interact with H2AFZ,[15] Survivin[16] and CDCA8.[17] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast.[18]

References

  1. [1]
    GRCh38: Ensembl release 89: ENSG00000149503 – Ensembl, May 2017
  2. [2]
    GRCm38: Ensembl release 89: ENSMUSG00000024660 – Ensembl, May 2017
  3. [3]
    "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. [4]
    "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. [5]
    Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci. 98 (4): 443–61. doi:10.1242/jcs.98.4.443. PMID 1860899.
  6. [6]
    Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma. 110 (2): 65–74. doi:10.1007/s004120100130. PMID 11453556. S2CID 13778759.
  7. [7]
    "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".
  8. [8]
    Sasai, Kaori; Katayama, Hiroshi; Hawke, David H; Sen, Subrata (2016-01-01). "Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex". PLOS ONE. 11 (6): e0157305. Bibcode:2016PLoSO..1157305S. doi:10.1371/journal.pone.0157305. ISSN 1932-6203. PMC 4917241. PMID 27332895.
  9. [9]
    Li, Xiangyu; Sakashita, Gyosuke; Matsuzaki, Hideki; Sugimoto, Kenji; Kimura, Keiji; Hanaoka, Fumio; Taniguchi, Hisaaki; Furukawa, Koichi; Urano, Takeshi (2004-11-01). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry. 279 (45): 47201–47211. doi:10.1074/jbc.m403029200. ISSN 1083-351X. PMID 15316025.
  10. [10]
    Honda, Reiko; Körner, Roman; Nigg, Erich A (2003-08-01). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis". Molecular Biology of the Cell. 14 (8): 3325–3341. doi:10.1091/mbc.e02-11-0769. ISSN 1939-4586. PMC 181570. PMID 12925766.
  11. [11]
    Jeyaprakash, A. Arockia; Klein, Ulf R.; Lindner, Doris; Ebert, Judith; Nigg, Erich A.; Conti, Elena (2007-10-19). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together". Cell. 131 (2): 271–285. doi:10.1016/j.cell.2007.07.045. ISSN 0092-8674. PMID 17956729. S2CID 15199822.
  12. [12]
    Choo, K. H. Andy (1997). The centromere. Oxford [Oxfordshire]: Oxford University Press. ISBN 978-0-19-857780-5.
  13. [13]
    Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. doi:10.1096/fasebj.8.12.8088460. PMID 8088460. S2CID 14062246.
  14. [14]
    Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. doi:10.1093/hmg/8.7.1145. PMID 10369859.
  15. [15]
    Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". EMBO J. 22 (7): 1599–607. doi:10.1093/emboj/cdg160. PMC 152904. PMID 12660166.
  16. [16]
    Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Curr. Biol. 11 (11): 886–90. Bibcode:2001CBio...11..886W. doi:10.1016/S0960-9822(01)00238-X. PMID 11516652.
  17. [17]
    Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC 2172304. PMID 15249581.
  18. [18]
    Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Mol. Biol. Cell. 13 (4): 1132–43. doi:10.1091/mbc.01-07-0330. PMC 102257. PMID 11950927.

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR005635
This article incorporates text from the public domain Pfam and InterPro: IPR022006
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