Isaac Van Horne (January 13, 1754, Solebury Township, Pennsylvania – February 2, 1834, Zanesville, Ohio) was a member of the U.S. House of Representatives from Pennsylvania.

He was the eldest son of Bernard Van Horne and his first wife Sarah Van Pelt. He is descended from several Dutch families that came to America when New York was a Colony of the Netherlands. His father's family, sometimes spelled "van Hoorn", had earlier been identified with the European Noble Family of that name.

His paternal immigrant ancestor Christian Barentsen van Hoorn had a house lot at Wall Street and Broadway which is now part of Trinity Churchyard, and was Fire Marshal of Manhattan in the 1650s before moving to the area of Wilmington, Delaware. His grandmother, Alice Sleght Van Horn, may have been descended from Catherine Trico Rapalgie, one of whose daughters was reputed to be the first white child born in New Netherland. He was also the 3x-great grand nephew of Peter Stuyvesant (c.1612—1672).

He apprenticed as a carpenter and cabinetmaker and served was justice of the peace for Solebury Township in Bucks County of Pennsylvania for several years as well as coroner of Bucks County four years.

He married Dorothy Marple, the widow of Isaac Marple with whom she had already had two sons: John J. Marple and David J. Marple. Dorothy had 11 more children with Isaac:

• Dorothy Jane Van Horne, who married Peter van Woglom and later Isaac van Horne (a cousin)
• Sarah Van Horne
• Mary "Polly" Van Horne, who married Jeffrey Price, a widower with a daughter
• CPT Isaac Van Horne Jr., 2d Battle of Fort Mackinac during the War of 1812
• Samuel Van Horne
• Eliza Nann Van Horne, who married Dr. John E. Hamm (1776–1864), Marshall of Ohio during War of 1812, who with Isaac Van Horne, founded the White Glass Co. of Zanesville, Ohio in 1815, of which Isaac Van Horne was President.
• Patience Van Horne, who married Harry Safford (a silversmith in Putnam, OH)
• Cynthia Van Horne
• Bernard Van Horne, who was his successor as receiver of public monies at Zanesville in 1826
• Benjamin Franklin Van Horne
• Bvt. Maj. Joseph Jefferson Van Horne, who fought in the U.S.-Mexican War.

Van Horne died February 2, 1834, in Zanesville, Ohio. One of the bequests in his will was a donation to the American Colonization Society.

• United States Congress. "Isaac Van Horne (id: V000045)". Biographical Directory of the United States Congress.
• The Political Graveyard
• Our Van Horne and Culbertson Forebears. William R. Van Horne, Lancaster, PA, 1983.
• Y Bridge City "The Story of Zanesville and Muskingum County, Ohio". Norris F. Schneider, The World Publishing Co., 1950.
• "a Brief Memoir of the Life of General Isaac Van Horne". Zanesville Ohio, (undated) privately reprinted several times.