The biosynthesis of isoflavonoids involves several enzymes; These are:

Liquiritigenin,NADPH:oxygen oxidoreductase (hydroxylating, aryl migration), also known as Isoflavonoid synthase, is an enzyme that uses liquiritigenin (a flavanone), O₂, NADPH and H₊ to produce 2,7,4'-trihydroxyisoflavanone (an isoflavonoid), H₂O and NADP⁺.

• Biochanin-A reductase
• Flavone synthase
• 2'-hydroxydaidzein reductase
• 2-hydroxyisoflavanone dehydratase
• 2-hydroxyisoflavanone synthase
• Isoflavone 4'-O-methyltransferase
• Isoflavone 7-O-methyltransferase
• Isoflavone 2'-hydroxylase
• Isoflavone 3'-hydroxylase
• Isoflavone-7-O-beta-glucoside 6"-O-malonyltransferase
• Isoflavone 7-O-glucosyltransferase
• 4'-methoxyisoflavone 2'-hydroxylase

• 3,9-dihydroxypterocarpan 6a-monooxygenase
• Glyceollin synthase
• Pterocarpin synthase

• Flavonoid biosynthesis