John Walker (February 13, 1744 – December 2, 1809) was a public official from Virginia.

Walker was born in Virginia, the son of Dr. Thomas Walker. He received private education before attending the College of William and Mary, which he graduated from in 1764. He was a neighbor and classmate at William and Mary of Thomas Jefferson and they remained close friends until the elections of 1804-1805. In 1768, he was elected to the American Society.^[1] In 1772, he replaced his father as a representative of the House of Burgesses. He was in the Continental Army, serving in 1777 as an aide-de-camp to General George Washington, holding the rank of colonel. In 1780, he was elected as a delegate to the Continental Congress. He then studied law. When William Grayson died in 1790, Walker was appointed to the United States Senate to serve from March 31 to November 9 of that year, when a successor was elected by the Virginia General Assembly. Thomas Jefferson’s attempted seduction of his wife, which almost led to a duel, and was fodder for the Federalist Press, is discussed in Dumas Malone’s Jefferson the Virginian (Boston, Little Brown and Company, 1948 Appendix III pp 447-451).

Walker owned slaves.^[2] He died in 1809.