John Walker may refer to:

• John Walker (animator), television animator and director
• John Walker (Australian actor) (fl. 1990s), Australian comedic actor
• John Walker (curator) (1906–1995), director of the National Gallery of Art
• John Walker (film producer) (born 1956), animated film producer
• John Walker (filmmaker) (born 1952), Canadian filmmaker and cinematographer
• John Walker (musician) (1943–2011), born John Maus, member of the 1960s singing group The Walker Brothers
• John Walker (organist) (born 1941), recording artist
• John Walker (painter) (born 1939), nominee for the Turner Prize in 1985
• John Augustus Walker (1901–1967), Alabama Gulf Coast artist
• John Edward Walker, British-born, American painter and educator
• John Henry Walker (1831–1899), Canadian engraver and illustrator

• John Walker (RAF officer) (born 1936), former Chief of Defence Intelligence
• John Walker (Medal of Honor) (1845–?), American Indian Wars soldier and Medal of Honor recipient
• John Walker (officer of arms) (1913–1984), English officer of arms
• John Anthony Walker (1937–2014), American communications specialist convicted in 1985 of spying for the Soviet Union
• John C. Walker, Indiana physician and officer during the American Civil War
• John George Walker (1821–1893), general in the Confederate States Army during the American Civil War
• John Grimes Walker (1835–1907), United States Navy admiral
• John T. Walker (USMC) (1893–1955), United States Marine Corp general

• John Walker (inventor) (1781–1859), English chemist and inventor of the friction match in 1827
• John Walker (natural historian) (1731–1803), Scottish naturalist
• John Walker (programmer) (born c. 1950-2024), one of the designers of AutoCAD
• John Charles Walker (1893–1994), American agricultural scientist
• John E. Walker (born 1941), British chemist, winner of the 1997 Nobel Prize
• John Francis Walker (1839–1907), British geologist
• John James Walker (1825–1900), British mathematician
• John M. Walker (1907–1990), American physician and investment banker
• John Walker (horticulturist) (1893–1991), Canadian plant breeder

• John Walker (grocer) (1805–1857), Scottish founder of John Walker & Sons and namesake of the Johnnie Walker whisky brand
• John Walker (Scottish cricketer) (1879–1953), Scottish businessman and director of John Walker & Sons
• John Brisben Walker (1847–1931), American entrepreneur and magazine publisher
• John Hardeman Walker (1794–1860), southeast Missouri landowner

• John Walker (archdeacon of Essex) (died 1588), Anglican archdeacon
• John Walker (archdeacon of Dorset) (1694–1780)
• John Walker (biographer) (1674–1747), English clergyman and ecclesiastical historian
• John Walker (scholar) (1692–1741), English classical scholar and Anglican archdeacon of Hereford
• John Walker (1769–1833), Church of Ireland cleric and academic who seceded as founder of a sect
• John M. Walker (bishop) (1888–1951), Episcopal bishop of Atlanta
• John Russell Walker (1837–1887), Anglican priest
• John T. Walker (bishop) (1925–1989), American Episcopal bishop of Washington
• John Walker (abolitionist) (1786–1845), Presbyterian minister in Ohio and Pennsylvania

• John Walker (industrialist) (1884–1932), Pittsburgh industrialist
• John Walker (journalist) (born 1977), British video game journalist
• John Walker (lexicographer) (1732–1807), English lexicographer, actor and philologist
• John Walker (philatelist) (1855–1927), British philatelist
• John Walker (numismatist) (1900–1964), Scottish numismatist
• John Walker (vaccinator) (1759–1830), English educational writer, physician, and advocate of vaccination
• John Walker (antiquary) (1770–1831), English antiquary
• John Brian Walker, British general practitioner
• John Walker, one of the Birmingham Six accused of bombings in England in 1974
• John A. Walker (art critic) (1938–2023), British art critic and historian
• John Clay Walker (1948–1985), American journalist
• John H. Walker (1872–1955), Scottish-born American labor unionist and politician
• John Walker (Protector of Aborigines), South Australian Protector of Aborigines, 1861–1868
• John Walker of Beanston (1670-1728), Scottish agricultural improver - introduced the fallow system to Scotland in 1690.^[1]

• John Walker, in Arthur Ransome's Swallows and Amazons series of novels
• John Walker, alter ego of U.S. Agent, a comic book superhero

• All pages with titles beginning with John Walker
• John Walker Lindh (born 1981), American captured as an enemy combatant in 2001, in Afghanistan, usually referred to by the press as John Walker
• Johnnie Walker (disambiguation)
• Jack Walker (disambiguation)
• Jon Walker (born 1985), American musician
• Jonathan Walker (disambiguation)