Keratin_8

Keratin 8

Keratin 8

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Keratin, type II cytoskeletal 8 also known as cytokeratin-8 (CK-8) or keratin-8 (K8) is a keratin protein that is encoded in humans by the KRT8 gene. It is often paired with keratin 18.

Quick Facts KRT8, Identifiers ...

Utility as an immunohistochemical stain

Antibodies to CK8 (e.g. CAM 5.2) can be used to differentiate lobular carcinoma of the breast from ductal carcinoma of the breast.[5] CAM 5.2, an antibody that reacts with an epitope found on both CK8 and CK18, is used in immunohistochemistry to demonstrate certain forms of cancer. In normal tissue, it reacts mainly with secretory epithelia, but not with squamous epithelium, such as that found in the skin, cervix, and esophagus. However, it also reacts with a range of malignant cells, including those derived from secretory epithelia, but also some squamous carcinomata, such as spindle cell carcinoma. It is considered useful in identifying microscopic metastases of breast carcinoma in lymph nodes, and in distinguishing Paget's disease from malignant melanoma. It also reacts with neuroendocrine tumors.[6]

Keratin 8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.[7]

Interactions

Keratin 8 has been shown to interact with MAPK14,[8] Pinin[9] and PPL.[10]

Cytokeratin 8 staining in the human breast cancer cell line MCF-7

See also

References

  1. [1]
    GRCh38: Ensembl release 89: ENSG00000170421 – Ensembl, May 2017
  2. [2]
    GRCm38: Ensembl release 89: ENSMUSG00000049382 – Ensembl, May 2017
  3. [3]
    "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. [4]
    "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. [5]
    Moriya T, Kasajima A, Ishida K, et al. (March 2006). "New trends of immunohistochemistry for making differential diagnosis of breast lesions". Med Mol Morphol. 39 (1): 8–13. doi:10.1007/s00795-006-0309-8. PMID 16575508. S2CID 5978885.
  6. [6]
    Leong, Anthony S-Y; Cooper, Kumarason; Leong, F Joel W-M (2003). Manual of Diagnostic Cytology (2 ed.). Greenwich Medical Media, Ltd. pp. 177–178. ISBN 1-84110-100-1.
  7. [7]
    W. Jeffrey Allard; Jeri Matera; M. Craig Miller; et al. (October 2004). "Tumor Cells Circulate in the Peripheral Blood of All Major Carcinomas but not in Healthy Subjects or Patients With Nonmalignant Diseases". Clinical Cancer Research. 10 (20): 6897–6904. doi:10.1158/1078-0432.CCR-04-0378. PMID 15501967.
  8. [8]
    Ku, Nam-On; Azhar Salman; Omary M Bishr (March 2002). "Keratin 8 phosphorylation by p38 kinase regulates cellular keratin filament reorganization: modulation by a keratin 1-like disease causing mutation". J. Biol. Chem. 277 (13). United States: 10775–82. doi:10.1074/jbc.M107623200. ISSN 0021-9258. PMID 11788583.
  9. [9]
    Shi, J; Sugrue S P (May 2000). "Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus". J. Biol. Chem. 275 (20). UNITED STATES: 14910–5. doi:10.1074/jbc.275.20.14910. ISSN 0021-9258. PMID 10809736.
  10. [10]
    Kazerounian, Shideh; Uitto Jouni; Aho Sirpa (October 2002). "Unique role for the periplakin tail in intermediate filament association: specific binding to keratin 8 and vimentin". Exp. Dermatol. 11 (5). Denmark: 428–38. doi:10.1034/j.1600-0625.2002.110506.x. ISSN 0906-6705. PMID 12366696. S2CID 25735126.

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