In enzymology, a L-pipecolate oxidase (EC 1.5.3.7) is an enzyme that catalyzes the chemical reaction

L-pipecolate + O₂ ${\displaystyle \rightleftharpoons }$ 2,3,4,5-tetrahydropyridine-2-carboxylate + H₂O₂

Thus, the two substrates of this enzyme are L-pipecolate and O₂, whereas its two products are 2,3,4,5-tetrahydropyridine-2-carboxylate and H₂O₂.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is L-pipecolate:oxygen 1,6-oxidoreductase. Other names in common use include pipecolate oxidase, and L-pipecolic acid oxidase. This enzyme participates in lysine degradation.