2008 season

On October 16, 2007, it was announced in a press conference that the Gladiators would be relocating to Cleveland, Ohio. This would be the second arena football team to play be in the Cleveland area; the Cleveland Thunderbolts played at the Richfield Coliseum in Richfield, Ohio, for three seasons from 1992 to 1994. Former Cleveland Browns Quarterback Bernie Kosar was announced as the public face of the team, as well as the President of football operations and minority owner. The Gladiators would play their home games at Quicken Loans Arena, the home of the NBA's Cleveland Cavaliers and the AHL's Lake Erie Monsters.^[7] The team also moved back to the Eastern Division of the National Conference for the first time since 2003. The team would still be known as the Gladiators and continue to retain the team colors of red, silver, and black.^[8]

On March 3, the Gladiators played their inaugural game in Quicken Loans Arena, a 61–49 win over the New York Dragons. Raymond Philyaw, the team's quarterback, was named the offensive player of the game after throwing for five touchdowns. The team's first season in Cleveland was a successful one, as they went on to have a winning record of 9–7. On June 21, the Gladiators clinched a playoff berth with a 47–35 win against the Columbus Destroyers as well as home field advantage for the first round.

Cleveland won its first playoff game in 10 years with a 69–66 win over the Orlando Predators. Raymond Philyaw threw for 436 yards (the second-most yards in AFL postseason history) and eight touchdowns, receiver Robert Redd caught a franchise best 204 yards and four touchdowns, and Brandon Hefflin had two interceptions and recovered a key fumble late in the game to seal the victory. The following week, the Gladiators advanced to their first conference championship game in team history when they beat the Georgia Force in the divisional round 73–70. Raymond Philyaw had eight touchdown passes and Robert Redd and Otis Amey each caught three touchdowns in the win. The 61 points scored by both teams in the second quarter was also an AFL record for combined points in a quarter. The Gladiators then traveled to Philadelphia to face the Soul in the National Conference Championship game. Unfortunately, their season ended one game short of the Arena Bowl with a tough 70–35 loss.

2009: AFL suspends operations

The National Conference Championship would be the Gladiators' last game for almost two years. The Arena Football League canceled its 2009 season and it seemed that operations would be suspended indefinitely. However, the league reorganized as Arena Football 1 before purchasing the assets of the Arena Football League, therefore assuming the AFL's history, and the Gladiators were revived for the 2010 season, with owner Jim Ferraro and Bernie Kosar returning as team president and Quicken Loans Arena continuing to act as the team's home field. On December 23, 2009, former Grand Rapids Rampage coach Steve Thonn was named as new head coach of the Gladiators.^[9] Soon after, Arena Football 1 decided to re-adopt the Arena Football League name.

2010 season

During their first season in two years, the Gladiators hovered around .500 for the majority of the time. Highlights of the season included blowout victories over the Utah Blaze and the Iowa Barnstormers, but there were also some close losses attributed to poor special teams play. On July 17, during their 15th game of the season, the Gladiators were officially eliminated from playoff contention when their division rivals the Milwaukee Iron defeated the Orlando Predators. The Gladiators finished their 2010 campaign with a 7–9 record.

2011 season

The Gladiators returned in 2011 with Steve Thonn once again serving as head coach. John Dutton also returned as the team's starting quarterback. The Gladiators started their 2011 campaign successfully, upsetting the Spokane Shock, the defending ArenaBowl Champions, at their own arena. However, this victory was marred by an Achilles' heel injury to John Dutton in the final minutes, which would ultimately sideline him for the season. Fortunately, the team's backup quarterback Kurt Rocco performed well in his first Arena football start as the Gladiators 3–0. In their home opener, wide receiver Troy Bergeron set a Gladiator franchise record with six touchdown catches during the game. Unfortunately, the Gladiators were not able to post their best start in franchise history, losing the next two weeks. Injuries to Rocco during midseason led to third-string quarterback and Cleveland-area Case Western University alum Dan Whalen mounting second half comebacks. However, Rocco would eventually retake the starting position for the rest of the season.

Through 2011, the Gladiators were very strong at home but often mediocre on the road. Though they finished with a 7–1 record at Quicken Loans Arena, they were only 3–6 in away games. But the season ultimately ended with success, as the team beat their newest rival, the Pittsburgh Power, to clinch the American Conference East Division and guarantee a spot in the playoffs. This was the second time in three seasons that Gladiators will enter the playoffs while in Cleveland, as well as the first time they have clinched the East Division.

The team entered the playoffs with a 10–8 record to face the Georgia Force in the first round. Despite playing at home, the Gladiators played poorly in the second half and were eliminated from contention, losing 50–41.

2012 season

On January 17, 2012, the Gladiators were sold to Cleveland Cavaliers and Lake Erie Monsters owner Dan Gilbert.^[10] With John Dutton back as the starting quarterback, the Gladiators began their 2012 campaign successfully, reaching a record of 6–3 at the midway point of the season. However, the team then went on a five-game losing streak, which included some controversy when on June 8, 2012, the Gladiators lost by forfeit to the Pittsburgh Power. This was the first ever game forfeited in AFL history, due to the Gladiators (representing the AFL player's union) going on strike to protest on-field safety concerns and contract disputes with AFL management.^[11] Though the Gladiators would end the streak with a Week 16 win against division rival Milwaukee, two more losses against Orlando and Utah officially eliminated the team from playoff contention. The Gladiators finished the 2012 season at 8–10, second place in the East Division.

2013 season

The Gladiators' 2012–2013 offseason saw the departure of veteran quarterback John Dutton, who had been with the team for the past three seasons. The team's fifth season in Cleveland was a struggle, as the Gladiators never were able to have any long sustained success over the course of the year, finishing at 4-14 (tied with Pittsburgh for the worst record in the entire Arena League).

2014 season

The Gladiators began the 2014 season winning their first nine games, more than doubling their entire win total from the previous season (four). They didn't lose a game until being defeated by the Pittsburgh Power 48–34 on May 31 – more than two months into the season. On Saturday June 7, 2014, the Gladiators set a team record for points scored in a game when they defeated the Iowa Barnstormers 86–49.^[12] On July 12, 2014, following their 50–47 overtime win over the San Antonio Talons, the Gladiators clinched the East Division Championship, and the #1 seed in the American Conference for the 2014 playoffs.^[13] On July 26, 2014, the Gladiators set an AFL record for wins in a single season by going 17–1 after defeating the Tampa Bay Storm, 56–49. This gave the Gladiators home field advantage throughout the 2014 AFL playoffs.^[14][15] On August 2, 2014, the Gladiators defeated the Philadelphia Soul, 39–37, on a last second field goal by Aaron Pettrey in the first round of the AFL playoffs to advance to the American Conference finals.^[16] On August 10, 2014, the Gladiators defeated the Orlando Predators 56–46 to win the American Conference Championship, and advance to the ArenaBowl for the first time in franchise history. In ArenaBowl XXVII, the Gladiators were defeated by the Arizona Rattlers, 72–32.^[17]

2015 season

The Gladiators opened the 2015 season by winning their first two games; however, on April 11 in an ArenaBowl XXVII rematch, the Gladiators lost to the Arizona Rattlers 49–41 in their home opener. Overall, the Gladiators were not able to replicate the success of 2014, finishing with a regular season record of 8–10, second in the East Division. This overall record did qualify for the 2015 AFL playoffs, drawing the East Division Champion Philadelphia Soul in the first round. The Gladiators' season ended on August 15 when they lost to Philadelphia 47–35 in the first round of the AFL playoffs.

2016 season

For the 2016 season, the AFL featured eight teams, due to a number of teams either folding or moving to other indoor football leagues. As part of the restructuring, the Gladiators were moved from the American to the National Conference, so as to give each conference four teams. They finished the regular season at 7–9, third place in the National Conference.

In the first round of the AFL playoffs, the Gladiators rallied and defeated the Los Angeles KISS 56–52 after trailing the bulk of the game. The Gladiators then lost to the Arizona Rattlers 82–41 in the National Conference championship game, ending their season.

2017 season

In 2017, the AFL was reduced from eight teams down to five, due to teams folding or transferring to other leagues. With only five teams, the AFL essentially played a round-robin style 14 game schedule, with the top four teams at the end of the season advancing to the playoffs. The Gladiators finished at 5–9, clinching third place and a playoff spot. Cleveland would go on to lose to the #2 seed Tampa Bay Storm 73–59 in the AFL semifinals to end their season.^[18]

Suspension of operations: 2017

In September 2017, it was announced that Quicken Loans Arena would be undergoing significant renovations during the 2018 and 2019 basketball offseason so as not to conflict with its primary tenant, the NBA's Cleveland Cavaliers.^[19] However, as the AFL season runs into the basketball offseason, the Gladiators had to take a two-year leave of absence beginning in November 2017 with hopes to return for the 2020 season.^[20]

When the renovations were completed, the league suspended all city-based team operations for at least the 2020 season putting the Gladiators' plans on hold.^[21] The AFL filed for bankruptcy November 27, 2019, and ceased operations.