Margaret of Bohemia (29 September 1373 – 4 June 1410) was the younger daughter of Holy Roman Emperor Charles IV and his fourth wife Elizabeth of Pomerania. Her siblings included Anne of Bohemia and Holy Roman Emperor Sigismund.

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In 1381, Margaret married John III, Burgrave of Nuremberg. The marriage only produced one child:

• Elizabeth of Nuremberg (1391–1429), who married Eberhard III, Count of Württemberg, and had issue.

Margaret died in 1410, aged thirty-six, and her husband died ten years later in 1420; he did not remarry after Margaret's premature death.

Her daughter Elizabeth gave birth to a granddaughter, also called Elizabeth. The younger Elizabeth was engaged to Albert III, Duke of Bavaria, but instead married John III of Werdenberg.