Duchess of Parma

The marriage took place on 11 October 1628 in Florence. The wedding celebrations were accompanied by much spectacle and pageantry, including the performance of Marco da Gagliano's opera La Flora, composed especially for the occasion. To welcome the couple back to Parma, Mercury and Mars, with music by Claudio Monteverdi and text by Claudio Achillini was performed in the Teatro Farnese.

The years in which the couple ruled over Parma were marked by the plague of 1630 and the contrast between the splendour of the court and the tax burden to which they had subjected their subjects. The tax money was used to improve Odoardo's armies. The Duke also had a pro-French policy.

According to portraiture and the sources of the time, Margherita was a beautiful woman, as well as amiable and well-educated. Unlike his predecessors, the duke was very devoted and loyal to her and there were never any sources that claimed Odoardo had illegitimate children. ^{[citation needed]}

Regency

On 11 September 1646, Odoardo died. Their eldest son, Ranuccio was not yet old enough to rule the Duchy alone so his mother, Margherita acted as regent until her son was old enough to govern alone two years later. She served in co-regency with her former brother-in-law, her son‘s uncle, Francesco Maria Farnese. In 1648, her son was declared of legal majority and thus ended her regency.

Margherita outlived at least four of her children. She died in Parma on 6 February 1679.