In enzymology, a methionine-glyoxylate transaminase (EC 2.6.1.73) is an enzyme that catalyzes the chemical reaction

L-methionine + glyoxylate ${\displaystyle \rightleftharpoons }$ 4-methylthio-2-oxobutanoate + glycine

Thus, the two substrates of this enzyme are L-methionine and glyoxylate, whereas its two products are 4-methylthio-2-oxobutanoate and glycine.

This enzyme belongs to the family of transferases, specifically the transaminases, which transfer nitrogenous groups. The systematic name of this enzyme class is L-methionine:glyoxylate aminotransferase. Other names in common use include methionine-glyoxylate aminotransferase, and MGAT.