Middleton and Prestwich was a parliamentary constituency centred on the Middleton and Prestwich districts of Greater Manchester. It returned one Member of Parliament (MP) to the House of Commons of the Parliament of the United Kingdom.

The constituency was created for the 1918 general election, and abolished for the 1983 general election, when it was partially replaced by the new constituencies of Heywood and Middleton and Bury South. Its member from 1974 to the seat's abolition was Jim Callaghan, who happened to share his name with the Labour Prime Minister.

It was an unusual constituency, because Middleton and Prestwich were physically separated by Heaton Park, a large green area bequeathed to Manchester City Council, and had nothing whatsoever in common. Prestwich was a well established middle class suburb with a large Jewish minority, and during the inter-war years boasted several millionaires. Middleton, on the other hand, was greatly expanded by a large Manchester overspill council estate, and at one point during the 1950s, Prestwich had no Labour councillors, while Middleton had no Conservatives. The new constituency of Heywood and Middleton in 1983 resolved this mismatch by linking together the two adjacent towns in the Rochdale borough, and was held by Labour right up to 2019. Prestwich joined neighbouring towns Radcliffe and Whitefield in the Bury Council area to become Bury South which was gained by the Conservatives until 1997 when it was lost to Labour, who held it up to 2019 when it was regained by the Conservatives. The 2018 Boundary Commission Review, which aimed to reduce the number of MPs from 650 to 600, and was subsequently shelved, had proposed to restore the Prestwich and Middleton seat.

1918–1950: The Borough of Middleton, and the Urban Districts of Chadderton and Prestwich.

1950–1983: The Boroughs of Middleton and Prestwich, and the Urban District of Whitefield.