Miloslav Holub (27 February 1915 – 12 March 1999) was a Czech actor, perhaps best known for his appearances (ranging from leads to cameo roles) in five films by Karel Zeman. He was also occasionally billed as Miroslav Holub.

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Miloslav Holub should not be confused with the Czech poet Miroslav Holub.

• The Trap (1950)
• Operation B (1952)
• The Tank Brigade (1955)
• The Fabulous World of Jules Verne (1958)
• The Slinger (1960)
• The Fabulous Baron Munchausen (1961)
• A Jester's Tale (1964) (as Miroslav Holub)
• The Stolen Airship (1967)
• On the Comet (1970)
• The Ear (1970)
• The Key (1971)