| Portfolio | Name | Political party | In Office |
| Prime Minister | Petr Pithart | OF, later OH | 29 June 1990 – 2 July 1992 |
| Deputy Prime Minister | Milan Lukeš | OF, later OH | 29 June 1990 – 2 July 1992 |
| František Vlasák | OF | 29 June 1990 – 31 May 1991 |
| Antonín Baudyš | KDU-ČSL | 29 June 1990 – 2 July 1992 |
| Jan Stráský | ODS | 1 June 1991 – 2 July 1992 |
| Minister of Finances | Karel Špaček | OF, later OH | 29 June 1990 – 2 July 1992 |
| Minister of Justice | Leon Richter | Independent, later OH | 29 June 1990 – 8 January 1992 |
| Jiří Novák | ODS | 8 January 1992 – 2 July 1992 |
| Minister of Environment | Bedřich Moldan | OF later ODS | 29 June 1990 – 24 January 1991 |
| Ivan Dejmal | KDS | 24 January 1991 – 2 July 1992 |
| Minister of Industry | Jan Vrba | Independent, later OH | 29 June 1990 – 2 July 1992 |
| Minister of Trade and Travel | Vlasta Štěpová | OF, later OH | 29 June 1990 – 2 July 1992 |
| Minister of Agriculture | Bohumil Kubát | OF, later ODA | 29 June 1990 – 2 July 1992 |
| Minister of Education | Petr Vopěnka | KDS | 29 June 1990 – 2 July 1992 |
| Minister of Interior | Tomáš Hradílek | OF | 29 June 1990 – 15 November 1990 |
| Tomáš Sokol | OF, later OH | 15 November 1990 – 2 July 1992 |
| Minister of Labour and Social Affairs | Milan Horálek | OF, later OH | 29 June 1990 – 2 July 1992 |
| Minister of Health | Martin Bojar | OF, later OH | 29 June 1990 – 2 July 1992 |
| Minister of Culture | Milan Uhde | OF, later ODS | 29 June 1990 – 2 July 1992 |
| Minister of Construction Industry | Ludvík Motyčka | KDU-ČSL | 29 June 1990 – 2 July 1992 |
| Ministry of State Control | Bohumil Tichý | HSD-SMS | 29 June 1990 – 27 February 1991 |
| Igor Němec | ODS | 27 February 1991 – 2 July 1992 |
| Minister of the Administration of National Property and its Privatization | Tomáš Ježek | OF, later ODA | 29 June 1990 – 2 July 1992 |
| Minister of Mechanical Engineering and Electrical Engineering | Miroslav Grégr | Independent | 29 June 1990 – 2 July 1992 |
| Minister of Economic Policy and Development | Karel Dyba | OF, later ODS | 29 June 1990 – 2 July 1992 |
| Minister without portfolio | Jaroslav Šabata | OF, later OH | 29 June 1990 – 2 July 1992 |
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