The Roman Catholic Diocese of Masbate (Latin: Dioecesis Masbatensis) is a diocese of the Latin Church of the Catholic Church in the Philippines. The seat of the bishop is the Cathedral Parish of Saint Anthony of Padua along Quezon Street, Masbate City.

The diocese has experienced no jurisdictional changes and is suffragan to the Archdiocese of Cáceres. Its patron is Saint Anthony of Padua.

With the other provinces of Bicol, Masbate once belonged to the Archdiocese of Cáceres. When Cáceres was elevated to the status of an archdiocese on June 29, 1951, Masbate became part of the newly erected Diocese of Sorsogon. Pope Paul VI created the Diocese of Masbate on March 23, 1968. It was formally inaugurated on September 25, 1968, with Msgr. Porfírio Rivera Iligan, a priest from the Archdiocese of Cáceres, as its first bishop.

The Vicar-General assists the bishop in the administrative duties over the diocese. The Offices of the Vicar General and the Episcopal Vicars temporarily ceased upon the vacancy of the episcopal see.

• Very Rev. Msgr. Anacleto Golimlim, P.A (†)
• Very Rev. Msgr. Arturo Lachica, H.P (†)
• Very Rev. Msgr. Claro Caluya III, H.P (1984–2009)
• Very Rev. Msgr. Ismael Misolas, P.C (2009)
• Very Rev. Fr. Eusebio Llonoso (2011–present)

• Masbate Cathedral
• Catholic Church in the Philippines