Anzoategui was born in Huntington Park, California, and raised in several communities around East Los Angeles. Their parents immigrated to the United States from Argentina and Paraguay. They are non-binary and use they/them pronouns.
Anzoategui attended college at Loyola Marymount University in California.
When Anzoategui first auditioned for scripted acting roles they were rarely cast even though they presented as femme at the time. They then began to write and perform autobiographical theater shows such as Ser and Catholic School Daze. Anzoategui was performing in theater productions when they were cast in East Los High. They portrayed Daysi Cantu, a character who is masculine of center.
They portrayed Eddy on Vida for the three seasons it ran 2018-2020. Eddy is the sensitive, butch widow of Vidalia, attempts to protect the business she co-owned with her wife when Vida's daughters return for the funeral. Anzoategui's performance on the show has been positively received by critics. Writing for LA Weekly, Lina Lecaro stated, "Powerfully played by L.A. native and longtime local theater actor Ser Anzoategui...Eddy...is in many ways the heart of the show. She conveys a subdued depth of pain, loss and hope while trying to forge a relationship with the young women, even as conflict emerges over how to honor the wishes of the woman who brought them together.
Anzoategui was nominated for “Best Supporting Actor” for their Vida performance at the 2019 Imagen Awards.
|2015–2016||East Los High||Daysi Cantu|
|2020||Disclosure: Trans Lives on Screen||Themself|
- Radish, Christina (2019-06-12). "'Vida' Season 2: Ser Anzoategui on Eddy's Journey and Being Non-Binary in Hollywood". Collider. Retrieved 2019-06-15.
- "What It's Like to Be a Non-Binary Latinx Actor in Hollywood". Remezcla. 2018-04-26. Retrieved 2019-06-15.
- "Vida's Non-Binary Latinx Actor Ser Anzoategui Already Knows Their Show Will Be Your New Favorite". Autostraddle. 2018-04-30. Retrieved 2019-06-15.
- Lecaro, Lina (2019-08-09). "Ser Anzoategui and the cast of Starz's Vida Convey Latinx Struggle in L.A." LA Weekly. Retrieved 2020-05-03.