TNO may refer to:

• Franco-Ténois, the widespread community of francophones who reside in the Northwest Territories
• Netherlands Organisation for Applied Scientific Research (Nederlandse Organisatie voor Toegepast Natuurwetenschappelijk Onderzoek), an independent research organization in the Netherlands
• Tamarindo Airport (IATA code: TNO), a private airport that serves Tamarindo, Costa Rica
• Tête nucléaire océanique, a French thermonuclear warhead
• Thai National Observatory, part of the National Astronomical Research Institute of Thailand
• TNO intestinal model, systems mimicking the digestive tract
• Trans-Neptunian object, any minor planet in the Solar System that orbits the Sun at a greater average distance than Neptune
• Texas and New Orleans Railroad, a railroad in Texas and Louisiana. Was bought by Southern Pacific in 1881, and merged with Southern Pacific in 1961.
• The New Order: Last Days of Europe, a mod for Hearts of Iron IV.

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