Titmouse, Inc. is an American animation studio based in Los Angeles, California founded in 2000 that develops and produces animated television programming, feature films, music videos, title sequences, commercials, and short films.

Founder and CEO Chris Prynoski began working at MTV in New York on shows such as Daria, Beavis and Butt-Head, and his own creation, Downtown, which was nominated for a primetime Emmy Award.^[3] In the early 2000s, Prynoski opened a small T-shirt company with his wife, Shannon Prynoski. However, upon getting more requests for cartoons than for T-shirts, they decided to abandon screen-printing in favor of animation. The Prynoskis moved to California, and opened the revamped Titmouse, Inc., an animation studio. Antonio Canobbio, who had worked with Chris Prynoski at MTV and both Prynoskis at Cartoon Network in L.A., was hired as creative director.^[4] Titmouse opened a games division in 2009.^[5]

Due to projects like Cartoon Network's Metalocalypse, Superjail! and The Venture Bros.^[6][7] the company expanded and opened a sister studio in New York City in 2010.^[8] The California studio later added a wholly owned subsidiary, Robin Redbreast, which was then unionized in order to produce Motorcity for Disney XD.^[9] Also it co-produced Randy Cunninhgam with Boulder Media.

The company has a YouTube channel, Rug Burn, launched in December 2012 with 6 Point Harness. Rug Burn was launched with a handful of shows.^[10]

In April 2016, Titmouse released its first feature-length film, the R-rated Nerdland, which stars Paul Rudd and Patton Oswalt.^[11]

In early 2020, Titmouse signed a multiyear production deal with Netflix.^[12]

In October 2020, Titmouse Vancouver became the first animation studio in Canada to join a union. Following a vote that captured 87% of the workforce, 98% of Titmouse Vancouver's workforce voted to join the newly founded Animation Guild IATSE Local 938.^[13] In response to this, some said that the issues raised by the workers of Titmouse were the same ones heard "from unrepresented animation workers everywhere" and that the unionization of the Titmouse workers acknowledges the contributions by animation workers to the industry.^{[14][15][lower-alpha 1]}

In January 2022, the employees of Titmouse New York formed a union with Animation Guild IATSE Local 839, the first to do so outside of Los Angeles County in more than 70 years. In the card check process, Titmouse NY employees showed more than 90% of support for the organizing effort. Following the union becoming public, Titmouse management chose to voluntarily recognize the union and agreed to negotiate with the Animation Guild in good faith.^[16]