USF may refer to:

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• University of Saint Francis (Indiana), Ft. Wayne, Indiana
• University of San Francisco, California
• University of South Florida, Tampa, Florida
• University of St. Francis, Joliet, Illinois
• University of Sioux Falls, South Dakota

• Universal Studios Florida, a theme park in Orlando, Florida

• Universal Subtitle Format
• Nintendo Ultra 64 Sound Format

• Ukrainian Shooting Federation, national governing body for shooting sports in Ukraine
• Universal Service Fund, a United States government program
• Upstream stimulatory factor, DNA-binding proteins regulating gene expression, see USF1 and USF2
• USAfrica Airways, defunct airline

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