2000–01 Ukrainian Second League

The 2000–01 Ukrainian Second League was the 10th season of 3rd level professional football in Ukraine.

Ukrainian Second League
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Champions(A) - Polissya Zhytomyr
(B) - Obolon Kyiv
(C) - Naftovyk Okhtyrka

The competitions were divided into three groups according to geographical location in the country – A is western Ukraine, B is southern Ukraine and Crimea, and C is eastern Ukraine.

The groups were won respectively by FC Polissya Zhytomyr, FC Obolon Kyiv and FC Naftovyk Okhtyrka.