The U.S. Senate Environment and Public Works Subcommittee on Transportation and Infrastructure is a subcommittee of the U.S. Senate Committee on Environment and Public Works.

• Transportation and Infrastructure Issues
  • Federal Highway Administration
  • Highway Program
  • Construction and maintenance of highways
  • Public works, bridges, and dams
  • General Services Administration
  • Public Buildings and improved grounds of the United States generally, including Federal Buildings in the District of Columbia
  • Green Building Standards
• Water Issues
  • Army Corps of Engineers (Civil Works)
  • Water Resources Development Act
  • National Dam Safety Program
  • Flood control and improvements of rivers and harbors, including environmental aspects of deepwater ports
  • Water resources
• Regional Economic Development
  • Economic Development Administration
  • Appalachian Regional Commission
  • Northern Border Regional Commission
  • Delta Regional Authority
  • Mississippi River Commission
• Transportation
• Federal Highway Administration

During committee organization for the 110th Congress, the subcommittee's responsibility over transportation safety and security issues was transferred to a new Subcommittee on Transportation Safety, Infrastructure Security, and Water Quality.