In enzymology, a vanillin dehydrogenase (EC 1.2.1.67) is an enzyme that catalyzes the chemical reaction

+ NAD⁺ + H₂O ${\displaystyle \rightleftharpoons }$ + NADH + H⁺

The 3 substrates of this enzyme are vanillin, NAD⁺, and H₂O, whereas its 3 products are vanillate, NADH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is vanillin:NAD+ oxidoreductase. This enzyme participates in 2,4-dichlorobenzoate degradation.