The following is a list of numbered roads in the Regional Municipality of Waterloo, Ontario. Regional roads are maintained by the Waterloo Region Transportation Department, and highways are maintained by the Ontario Ministry of Transportation, except where they are locally maintained as Connecting Links.

The following is a list of provincially maintained highways in Waterloo Region. Communities are ordered by where the route encounters them (either from south to north or from west to east).

More information Route, Type ...

Route	Type	Local Name(s)	Western/Southern Terminus	Eastern/Northern Terminus	Communities	Comments
Highway 7	Partial controlled access highway	Conestoga Parkway, Waterloo Regional Road 55 (Victoria Street)	Waterloo Regional Road 1 (to Stratford)	Woolwich Guelph Townline Road (to Guelph)	New Hamburg, Kitchener, Breslau	Part of the Conestoga Parkway. Shares the route with Highway 8, becoming Highway 7/8, from Kitchener to Stratford.
Highway 8	Partial controlled access highway	Conestoga Parkway,	Highway 401	Waterloo Regional Road 1 (to Stratford)	Cambridge, Kitchener, New Hamburg	Part of the Conestoga Parkway. Shares the route with Highway 7, becoming Highway 7/8, from Kitchener to Stratford.
Highway 24	Arterial road	Brantford Highway	Lockie Road	Waterloo Regional Road 24	Cambridge	Becomes Waterloo Regional Road 24 (Water Street) near Cambridge city limits.
Highway 85	Full controlled access highway	Conestoga Parkway	Highway 7 (at Victoria Street)	Waterloo Regional Road 85	Kitchener, Waterloo	Part of the Conestoga Parkway. Becomes Waterloo Regional Road 85 (Arthur Street) near Waterloo city limits. Only highway entirely within the region.
Highway 401	Full controlled access highway		Waterloo Regional Road 70	Waterloo Regional Road 33	Kitchener, Cambridge

Close

• "Regional Roads By Road Number" (PDF). Region of Waterloo. November 2021.
• The Regional Municipality of Waterloo (June 2020). "Region base map (scale 1:100,000)" (PDF).