Prescott to Elk Mound

WIS 29 begins at its western terminus at U.S. Highway 10 (US 10), on the north side of Prescott. From this point, WIS 29 is concurrent with WIS 35 northeast to River Falls, passing the University of Wisconsin–River Falls campus. At the junction with WIS 65, WIS 35 turns northbound with WIS 65 while WIS 29 continues east. WIS 29 joins briefly with US 63 west of Spring Valley^[2] and turns eastbound upon its split one mile (1.6 km) south to head into Spring Valley.^[3] Upon leaving Spring Valley and Pierce County, Wisconsin, the highway heads northwardward into St. Croix County and turns east towards Menomonie. WIS 29 does not pass through any municipalities while in this county, but it does cross WIS 128.^[4]

Upon entering Dunn County, the route passes through rural terrain and enters Menomonie's west side as Hudson Road. The highway turns northward onto Main Street and passes west and north of the University of Wisconsin–Stout campus. WIS 29 turns east onto Stout Road along with US 12 and leaves the city to the east.^[5] US 12/WIS 29 proceeds east for about nine miles (14 km) and junctions with Interstate 94 (I-94). This interchange is the westernmost point of the multi-lane WIS 29 corridor.^[6] It is also the westernmost point of WIS 29 as a backbone route in Corridors 2020.^[7]

Elk Mound to Wausau

West of County Highway T (CTH-T), the highway is an expressway. It passes into the Chippewa Valley metropolitan area and south of Chippewa Falls as a freeway and crosses US 53 at a cloverleaf interchange in Lake Hallie.^[8] WIS 29 junctions with the southern terminus of WIS 178 southeast of Chippewa Falls then passes to the south of Lake Wissota State Park before crossing under WIS 27 in Cadott. WIS 29 passes south of Boyd and Stanley before exiting the county to the east.^[8]

In Clark County, WIS 29 meets WIS 73 in Thorp. The two highways run concurrently and exit the village to the east, and WIS 73 splits to the south in Withee about 10 miles (16 km) east. WIS 29 continues east and passes south of Owen and Curtiss. At Abbotsford, WIS 29 and WIS 13 meet at a partial cloverleaf interchange between Abbotsford and Colby on the Clark–Marathon county line.^[8] Twelve miles (19 km) east of Abbotsford, WIS 29 and WIS 97 meet at a diamond interchange. This is a key route for travelers between Wausau and Marshfield. East of this junction, WIS 29 follows a straight course that passes Edgar and meets WIS 107 at a diamond interchange on the north side of Marathon City, approximately eight miles (13 km) west of Wausau. WIS 29 enters Wausau as a freeway, junctions with the western terminus of WIS 52 approximately 1⁄2 mile (0.80 km) west of US 51 at an eastbound off/westbound on style half-interchange. WIS 29 then immediately enters the US 51 interchange, and turns south onto and follows US 51 for five miles (8.0 km), bypassing the downtown area to the south.^[9]

Wausau to Green Bay

South of Wausau, WIS 29 turns off US 51 to the east at the northern terminus of I-39 and continues as freeway, passing through the villages of Rothschild and Weston. The freeway section ends at Ringle. As an expressway, the highway passes just south of Hatley and turns southeast for about four miles (6 km) before turning east again at the northern terminus of WIS 49.^[9]

The highway enters Shawano County about one mile (1.6 km) east of this junction.^[9] WIS 29 passes north and around Wittenberg. US 45 joins with the highway north of Wittenberg and the two routes run concurrently to where US 45 splits to the south at about two miles (3.2 km) east of the village. WIS 29 bypasses Tilleda and Thornton on the way towards Shawano. At Shawano, the expressway becomes a freeway at the junction with CTH-MMM (WIS 29 Business) and turns southeast to bypass Shawano to the south, diamond interchanges link the highway with WIS 22 and WIS 47 north/WIS 55 north as it pass south of the city. WIS 47 south and WIS 55 south follow WIS 29 east. WIS 47 splits to the south at the diamond interchange junction with WIS 117 south of Bonduel. WIS 29 (along with WIS 55) then returns to an expressway at the junction with CTH-BE (WIS 29's former route). WIS 55 splits to the south at the diamond interchange junction with WIS 160 at Angelica and WIS 29 heads southeast to the Brown County line.^[10]

About one mile (1.6 km) into Brown County, WIS 29 collects WIS 32 eastbound at the diamond interchange with WIS 156 and CTH-Y. WIS 29 very briefly passes through the northeast corner of Outagamie County and enters the village of Howard. The multilane expressway and the Backbone Corridor route end at Interstate 41/US 41.^[7][11] WIS 32 turns south onto I-41/US 41 as WIS 29 continues into Green Bay as Shawano Avenue.^[12]

Green Bay to Kewaunee

In downtown Green Bay, WIS 29 turns north onto South Monroe Avenue for four blocks, joining with WIS 54 and turns east onto Main Street, splitting from WIS 54 and joining US 141 to leave the city to the southeast. The highways pass over I-43 without an interchange. WIS 29 splits to the east from US 141 at about one mile (1.6 km) north of US 's southern terminus at I-43 in Bellevue. This provides the only access from I-43 to WIS 29. The highway passes through Poland and Henrysville as it exits Brown County to the east.^[11] In Kewaunee County, WIS 29 passes due east through Pilsen and north of Krok as a two-lane road. The highway ends in downtown Kewaunee at WIS 42 on the shores of Lake Michigan.^[13]