Main

• Wubbzy (voiced by Grey DeLisle in the US and Janet James in the UK) is a childish and friendly yellow mouse-like creature. He is small, cute and obsessed with his tail, on which he can bounce like a pogo stick.
• Widget (voiced by Lara Jill Miller in the US and Julie-Ann Dean in the UK) is a pink rabbit-like creature. She is a mechanical whiz with an incredibly heavy stereotypical Southern accent. She is a builder and engineer who has built all sorts of inventions.
• Walden (voiced by Carlos Alazraqui in the US and Wayne Forester in the UK) is a purple bear-like creature who speaks with an Australian accent. He is a polymath, brainiac, and scientist. His intellectualism and love for science are a common thread in the show.
• Daizy (voiced by Tara Strong in the US and Lynn Cleckner in the UK) is a cheerful dog-like creature who is Wubbzy's neighbor. As her name suggests, she loves flowers. She was introduced in Season 2.

Supporting

• Buggy, Huggy, and Earl (voiced by Grey DeLisle, Lara Jill Miller, Carlos Alazraqui respectively) are 3 friends who sometimes hang out with Wubbzy. Buggy is turquoise, Huggy is light blue, and Earl is orange.
• Kooky Kid (voiced by Grey DeLisle in the US and Janet James in the UK) is an orange-colored creature who appears when something bizarre happens stating that it's "kooky" while wiggling his fingers.
• Shine, Shimmer, and Sparkle (voiced by Beyoncé, Tara Strong, and Grey DeLisle respectively) are pop singers known as the Wubb Girlz. All 3 are light-blue colored. Shine is blonde, Shimmer is pink-haired, and Sparkle has green hair.

Recurring

• Ty Ty the Tool Guy (voiced by Ty Pennington) is an orange-colored resident appeared in the eponym episode, titled Ty Ty the Tool Guy.
• Policeman (voiced by Carlos Alazraqui) is a light orange-colored resident who is a police officer of Wuzzleburg.
• Chef Fritz (voiced by Carlos Alazraqui in the US and Wayne Forester in the UK) is an orange-colored chef who is a chef of Wuzzleburg.
• Madame Zabinga (voiced by Grey DeLisle) is a yellow-colored resident who is the only teacher of the local ballet school. She speaks with a French accent.
• Mayor Whoozle (voiced by Carlos Alazraqui) is the eccentricly-dressed mayor of Wuzzleburg.
• News Reporter (voiced by Carlos Alazraqui in the US and Wayne Forester in the UK) is a news reporter of Wuzzleburg. He is seen in the episode "Widget Gets the Blooey Blues"
• Miss Bookfinder (voiced by Grey DeLisle) is a librarian of the Wuzzleburg Library. She is first seen in the episode "A Little Help From Your Friends"
• Moo Moo the Magician (voiced by Carlos Alazraqui in the US and Wayne Forester in the UK) is a magician in Wuzzleburg. He first appears in "Magic Tricks" where he helps Wubbzy learn to do magic tricks.

International

This section does not cite any sources. (November 2020)

Wow! Wow! Wubbzy! has aired internationally. In Latin America, the series was aired initially on Discovery Kids Latin America from October 8, 2007, to 2016.^{[citation needed]}

It also aired on TG4 in Ireland, DR2 in Denmark, Baraem TV in Arab countries, Super RTL in Germany, Hop! Channel in Israel, and Discovery Kids in Latin America and Brazil. The series is shown on Canal Panda, Clan, and RTP2 in Portugal and Spain.

A British English dub was aired on Nick Jr. from 2006 to 2013. A translated version of the series has been a popular addition to the "Cúla 4" children's programming lineup on the Irish language television channel TG4 since January 2011. The show has been dubbed in 15 languages and aired in over 100 countries around the world.^{[citation needed]}