The 2001 Categoría Primera A season, named Copa Mustang 2001 for sponsoring purposes, was the 54th season of Colombia's top-flight football league. América de Cali were the defending champions and won their eleventh title and second in a row by beating Independiente Medellín in the finals.^[1]

The season was split into four stages: the first two stages were the Apertura and Finalización tournaments, in which the 16 teams were first divided into two groups of eight teams, playing seven games, and then all teams in the league played each other once for a total of 22 matches. The winners of each tournament earned a berth into the 2002 Copa Libertadores. The third stage was the semifinal round, which was played by the best eight teams of the aggregate table of both tournaments, who were divided into two groups of four according to their position in the aggregate table: odd-ranked teams made up Group A, while even-ranked ones made up Group B. The winners of each group played the double-legged finals to decide the champions of the season, who also qualified for the Copa Libertadores.

The Torneo Apertura (also known as Copa Mustang I) began on 11 February and ended on 1 July.

The Torneo Finalización (also known as Copa Mustang II) began on 5 August and ended on 11 November.

An aggregate table known as Reclasificación including the games of both tournaments (Apertura and Finalización) was used to determine the eight teams that would advance to the next stage of the tournament. The top eight teams in this table at the end of the Torneo Finalización advanced to the semifinals.

The third stage of the tournament consisted of two groups of four teams, with Group A including teams in odd-numbered positions and Group B including teams in even-numbered positions. The winners of each group qualified for the Finals.