Aldehyde-stabilized_cryopreservation

Aldehyde-stabilized cryopreservation

Aldehyde-stabilized cryopreservation

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Aldehyde-stabilized cryopreservation is a new technique for cryopreservation first demonstrated in 2016 by Robert L. McIntyre and Gregory Fahy at the cryobiology research company 21st Century Medicine, Inc. This technique uses a particular implementation of fixation and vitrification that can successfully preserve a rabbit brain in "near perfect" condition at −135 °C, with the cell membranes, synapses, and intracellular structures intact in electron micrographs.[1] The technique has finally won the Small Animal Brain Preservation Prize of the Brain Preservation Foundation.[2][3] The cryopreserved brain was rewarmed and no serious degradation was found to have occurred; the brain structure under electron microscopic evaluation after rewarming remained well-preserved.[4][5] Although this technique has not yet lead to a successful revival of a cryopreserved brain, some researchers see this technique as providing promising directions for future research.[6]

See also


References

  1. McIntyre RL, Fahy GM (December 2015). "Aldehyde-stabilized cryopreservation". Cryobiology. 71 (3): 448–458. doi:10.1016/j.cryobiol.2015.09.003. PMID 26408851.
  2. Claire Maldarelli (9 February 2016). "Researchers Have Preserved an Entire Rabbit Brain". Popular Science. Archived from the original on 14 March 2016. Retrieved 20 March 2016.
  3. Michael Shermer (1 February 2016). "Can Our Minds Live Forever?". Scientific American. Archived from the original on 22 March 2016. Retrieved 20 March 2016.
  4. Ekpo, Marlene Davis; Boafo, George Frimpong; Gambo, Suleiman Shafiu; Hu, Yuying; Liu, Xiangjian; Xie, Jingxian; Tan, Songwen (2022-06-09). "Cryopreservation of Animals and Cryonics: Current Technical Progress, Difficulties and Possible Research Directions". Frontiers in Veterinary Science. 9. Frontiers: 877163. doi:10.3389/fvets.2022.877163. PMC 9219731. PMID 35754544.



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